Plasmid_Backbone

Part:BBa_K1971011:Design

Designed by: KE GUAN YU   Group: iGEM16_BHU_China   (2016-10-20)


psb1c3 backbone with BsaI site


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 2056
    Illegal suffix found at 8
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2056
    Illegal SpeI site found at 9
    Illegal PstI site found at 23
    Illegal NotI site found at 16
    Illegal NotI site found at 2062
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2056
    Illegal XhoI site found at 1040
    Illegal XhoI site found at 1932
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2056
    Illegal suffix found at 9
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2056
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2071
    Illegal SpeI site found at 9
    Illegal PstI site found at 23
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 2


Design Notes

None


Source

psb1c3 circulized plasmid

References